"Isoeugenol is a very effective anesthetic in fish, used for example during the treatment of fish against parasites. However, it was previously unclear what the anesthetic effect of isoeugenol is based on. We were able to demonstrate that isoeugenol mainly affects the sensory systems of fish, i.e. it prevents them from taking up information from the environment. Even comparatively small amounts of isoeugenol massively reduce vision, significantly higher doses are required to switch off hearing. However, all sensory systems recover quickly and completely after isoeugenol administration," says first author Dr. Peter Machnik, a research associate at the Department of Animal Physiology. The new findings do not support the assumption that isoeugenol blocks sodium channels in the central nervous system of fish and thus prevents the processing of sensory information in the brain. Primarily, the active ingredient acts as a local anesthetic. Only when high doses were administered the Bayreuth researchers did also detect an effect on central neurons, but this effect was not reconciled with the idea of a blockade of sodium channels.
The authors of the study consider isoeugenol to be an anesthetic that, when used in an aimed manner, is suitable for reducing stress and pain in fish, both in scientific research and in fish farming. It has not only an anesthetic but also a sedative effect on the fish. However, isoeugenol has proven unsuitable as an anesthetic during invasive procedures or for calming fish while their sensory systems are being studied, for example in studies on sensory systems – if only because the present study has shown that the same dosage has very different effects on different fish and the desired reducing effect is not always guaranteed.
"We recently presented the study at the meeting of the German Neuroscience Society in Göttingen. Our results on the effects of isoeugenol met with a great deal of scientific interest. They were seen as a valuable contribution to future animal welfare, because isoeugenol is now used in many fish farms worldwide without precise knowledge of its effects and of the necessary dosages," says Prof. Dr. Stefan Schuster, Chair of Animal Physiology at the University of Bayreuth.
Publication:
Peter Machnik, Nastaran Biazar, Stefan Schuster: Recordings in an integrating central neuron reveal the mode of action of isoeugenol. Communications Biology (2023), DOI: https://doi.org/10.1038/s42003-023-04695-4
